摘要
采用电镜细胞化学技术对栉孔扇贝 (Chlamysfarreri)外套膜中的酸性磷酸酶(ACP)和碱性磷酸酶 (AKP)进行了定性定位研究。结果表明 ,外套膜的所有上皮细胞和结缔组织中部分血细胞呈ACP强阳性。上皮细胞中高电子密度阳性颗粒多为圆形 ,直径30 0～ 4 5 0nm ;内质网也呈ACP强阳性 ;微绒毛等呈弱阳性。血细胞中次级溶酶体内有数目和大小不同的ACP强阳性颗粒 ;内质网等内膜系统呈ACP弱阳性。外套膜的所有上皮细胞和血细胞均呈AKP强阳性。AKP阳性颗粒常沿着细胞膜分布 ;内质网等内膜系统呈AKP强阳性 ;上皮细胞中一种近圆形直径约 2 5 0～ 4 2
The acid phosphatase and alkaline phosphatase in the mantle of scallop Chlamys farreri were studied by cytochemical localisation with electron microscope All of the epithelial cells and a part of haemocytes in the connective tissue of the mantle present strong ACP positivity Most of the positive granules with high electron density in the epithelial cells are round and the diameter are 300～450nm The endoplasmic reticulum in the epithelial cells present strong ACP positivity too The microvilli are weakly positive of ACP There are different number and sized strong ACP positive granules in the secondary lysosomes of the haemocytes The endoplasmic reticulum in the haemocytes are weakly positive of ACP All of the epithelial cells and haemocytes in the connective tissue of the mantle present strong AKP positivity The AKP are located on plasma membrane, along which distribute granules of high electron density scatteringly The endoplasmic reticulum present strong AKP positivity A kinds of strong AKP positive granules approximate round in the cytoplasm of the epithelial cells are about 250～420nm in diameter
出处
《高技术通讯》
EI
CAS
CSCD
2002年第5期99-102,共4页
Chinese High Technology Letters
基金
国家 973计划项目 (G19990 12 0 0 5 )
山东省优秀中青年科学家奖励基金项目资助
