流行性出血热病毒感染家兔后外周血液淋巴细胞内病毒抗原反应的动态观察

Sequential Observation of Viral Antigen in Lymphocytes of Peripheral Blood of Rabbits Infected with EHFV-J10 Strain

家兔感染EHFV-J10株后,用PAP方法、单克隆荧光抗体法及间接IFA检测了外周血淋巴细胞内病毒抗原反应,发现:感染后第5天,病毒抗原开始在淋巴细胞胞浆内出现,第6～8天抗原反应性较强,第10天后阳性抗原逐渐消失。结果还表明,用PAP法对淋巴细胞内EHFV抗原检出的阳性动物数明显高于单克隆荧光抗体法和IFA法,且抗原检出时间早于后2种方法。

Rabbits inoculated with EHFV-J10 strain showed that viral antigen were detected in the cytoplasm of the peripheral blood lymphocytes by PAP method,monoclonal antibody method and indirect IFA in the period of 5～10 days post-infection.The stronger antigen reaction occured between 6～8 days post-infection and viral antigen disappeared after the 11th infection day. Results of the experiment also showed that PAP method is more sensitive in detecting viral antigen of lymphocyted than IFA.