HEV嵌合抗原的克隆表达及在抗-HEV ELISA试剂中的应用

Cloning, Expression and Applocation of A Chimeric Antigen of Hepatitis E Virus(HEV)

通过反转录从戊肝病人粪便中克隆了戊型肝炎病毒（HEV）第二（ORF2）和第三（ORF3）读码框区的抗原决定簇基因,利用基因工程手段,在大肠杆菌中表达了含有上述二种抗原的融合蛋白,该融合蛋白具有良好的抗原性和特异性,以该融合蛋白为抗原制备的抗-HEV ELISA试剂检测中国药品生物制品检定所40份标准血清时,其阴性、阳性符合率分别达29/30、10/10。

Gene encoding HEV ORF2 ,ORF3 antigen epitopes was cloned by RT-PCR. Gene fusion containing the two genes was made and expressed in Escherichia coli , The chimeric antigen obtained showed high sensitivity and specificity when it was used as capture antigen in anti- HEV ELISA KIT.