摘要
用定量鲎基质显色法测定LaCl3结合内毒素 (LPS)的能力。通过ELISA、荧光定量RT PCR方法观察LaCl3对LPS诱导的小鼠腹腔巨噬细胞 (Mφ)肿瘤坏死因子 α(TNF α)分泌及TNF αmRNA表达的影响。通过小鼠死亡率观察LaCl3对LPS毒性的影响。结果显示LaCl3具有结合LPS ,抑制TNF α分泌及TNF αmRNA表达 。
The ability of LaCl 3 to bind lipopolysaccharide (LPS) was determined based on quantitative limulus test. The effect of LaCl 3 on the secretion of TNF α and the expression of TNF αmRNA in murine peritoneal macrophages stimulated by LPS was studied by ELISA and SYBR Green I fluorescence quantitative RT PCR method. The influence of LaCl 3 on the virulence of endotoxin was evaluated by observing the mortality rate of mouse challenged by LPS. The results indicate that LaCl 3 has functions of binding LPS, decreasing the toxicity of LPS, suppressing secretion of TNF α and expression of TNF αmRNA.
出处
《中国稀土学报》
CAS
CSCD
北大核心
2002年第2期190-192,共3页
Journal of the Chinese Society of Rare Earths
基金
国家自然科学基金资助项目 ( 398690 0 3)
关键词
稀土
氯化镧
内毒素
rare earths
lanthanum chloride
lipopolysaccharide
