益母草药材生物检定方法的研究(Ⅰ)——动物标准子宫模型建立和测定环境因素筛选

Study on the Biological Assay of Herba Leonuri(I)--Establishment of Standard UterusModels and Optimizing Uterus Environment Conditions

目的:研究益母草缩宫效应,建立动物标准子宫模型,优选影响益母草缩宫效应的环境因素。方法:通过比较益母草对小鼠、大鼠、家兔离体子宫的缩宫效应,确定益母草药材生物检定的标准子宫模型;运用正交试验,对影响益母草缩宫效应的主要环境因素进行筛选。结果:以动情前期大鼠离体子宫为标准子宫模型,益母草药材生物检定最佳环境条件为:子宫负荷1 g,浴液温度32℃,每 1000 ml子宫蓄养液CaCl_2和NaHCO_3含量分别为0.06g和0.25g。结论:优选后的条件,益母草缩宫效应明显,检定误差较小。

Objective:To study the contracting uterus effect of Herba Leonuri,establish the standard uterus models,and optimize the environment conditions which could influence Herba Leonuri actions on uterus.Methods: By comparing the contracting uterus effect of Herba Leonuri in mice, rats and rabbits, the standard uterus models was established.The optimum extra conditions of uterus were selected by orthogonal design methods.Results:The isolated rat uterus pre-oestrus was selected for the standard uterus, the optimum uterus environment conditions as follows: the pre-burden of uterus was 1.0 g, the temperature of uterus nutrition solution is 32t , the assay of CaCl2 and NaHCG3 per 1000 ml nutrition solution was respectively 0.06 g and 0.25 g. Conclusion: Under the selected conditions, the contracting uterus effect of Herba Leonuri were more obvious and the errors of bioassay were less smaller.