异丙酚对离体人支气管平滑肌的作用

The effect of propofol on isolated human bronchial smooth muscle

目的 观察异丙酚对离体人肺叶支气管平滑肌的作用。方法 离体人肺叶支气管取自胸外科肺叶切除术患者（n=10）,采用离体气管实验法,以乙酰胆碱（3μg·ml-1）激发,观察临床相关剂量的异丙酚（1、2、5、10、20μg·ml-1）对离体人肺叶支气管平滑肌的作用,脂肪乳剂作为对照。结果与对照组相比,1、2、5、10、20μg·ml-1的异丙酚显著减弱乙酰胆碱的平滑肌收缩反应（P值分别<0.05、<0.01、<0.01、<0.01、<0.01）。结论 临床相关剂量的异丙酚可有效地减弱乙酰胆碱介导的离体人肺叶支气管平滑肌的收缩反应.

Objective Propofol is now the most commonly used intravenous anesthetic. It has vasodilating effect. The purpose of this study is to determine its effect on bronchial smooth muscle. Methods Strips of bronchus (40x2cm2) were taken from the lung resected during lung surgery and suspended a bath filled with K-H solution. One end of the bronchus strip was fixed to the bottom of the bath and the other was attached to a tension transducer through a piece of wire. Bronchoconstriction was induced with acetylcholine added in K-H solution (3μg·ml-1). When peak bronchoconstriction was reached, propofol in fat emulsion was added culmulatively to the bath (1,2,5, 10,20μg·ml-1). The effect of fat emulsion was also examined as control. Results Propofol at concentrations of 1, 2, 5, 10 and 20(μg·ml-1 caused a significant decrease in the acetylcholine-induced bronchoconstriction in a dose dependent manner. Conclusions Propofol at clinically relevant concentrations significantly attenuates acetylcholine-induced bronchoconstriction in human.