P16蛋白表达与原发性食管鳞癌患者临床病理相关性研究

Research on correlation of the expression of p16 gene with clinicopathological factors in human esophageal squamous cell carcinoma

目的 探讨抑癌基因p16在食管鳞癌组织中的蛋白表达与临床、病理变化之间的关系。方法 应用改良SABC免疫组化法 ,对 5 4例手术切除的原发食道鳞癌患者的肿瘤组织蜡块进行p16基因蛋白表达产物的测定。结果 5 4例食管鳞癌病人中p16基因表达缺失 2 1例 ( 38.9% )。p16表达与肿瘤病理分级关系密切 ,p16表达缺失者肿瘤组织分化程度明显低于p16表达阳性者 (P <0 .0 0 0 1)。p16基因表达与区域淋巴结转移也有一定的相关性。结论 原发性食管鳞癌患者的p16基因蛋白表达缺失较常见 ,且与肿瘤组织的分化程度密切相关 ,与淋巴结转移也有一定的关系。利用SABC免疫组化法检则p16基因的表达情况 ,可作为原发性食管鳞癌的辅助诊断手段 ,同时也为选择恰当的治疗方案提供了重要的信息。

Objective To evaluate the correlation between expression of p16 protein and various clinicopathological parameters in patients with esophageal squamous cell carcinoma(SCC) treated by esophagetomy and chemotherapy.Methods Loss of expression of p16 gene in human primary esophageal SCC was examined in 54 paraffin embedded tumor tissues using a polyclonal antibody against p16 protein by means of modified SABC immunohistochemical method.Results A total 54 esophageal SCC patients were examined,with 21(38.9%) found to have lost wild type p16 expression.The tumor differentiation in patients with loss of p16 expression was significantly poorer than that with p16 expression (P<0.001).There was also a correlation between the gene expression and regional lymph node metastasis.Conclusion Loss of p16 protein in human primary SCC of esophagus is a frequent event and correlates significantly with the tumor differentiation.Immunohistochemical evaluation of p16 expression may provide important information for selecting more suitable treatment for patients with esophageal SCC.