应用PCR技术检测胃炎患者Hp的Ure基因和CagA基因

Studies on the Ure Gene and CagA Gene of Hp by the PCR Technique in the Gastritis Patients

目的 :探讨浅表性胃炎、胃溃疡、萎缩性胃炎与幽门螺杆菌 (Hp)的尿素酶 (Ure)基因和细胞毒素蛋白 (Cag A)基因的关系。方法 :应用 PCR技术 ,选择与 Hp的 U re基因和 Cag A基因 3'末端核苷酸互补的两对引物 ,进行 PCR扩增 ,检测不同胃炎患者的 U re基因和 Cag A基因。结果 :胃溃疡患者 Ure阳性率 4 5 .7% ,Cag A阳性率 2 8.5 % ;浅表性胃炎 Ure阳性率 31.4 % ,Cag A阳性率 2 0 .0 % ;萎缩性胃炎 Ure阳性率 2 0 .0 % ,Cag A阳性率 5 3.3%。结论

Objective:To study the relationship between the atropic gastric,the surface layer gastritis and the gastric ulcen and the Ure gene and CagA gene of HP.Methods:Select two pairs of primer to pair 3-end of Ure gene and CagA gene.PCR assay was used to determine the Ure gene and CagA gene in the gastritis patients. Results:The positive rate of Ure gene is 45.7% and the positive rate of CagA gene is 28.5% in the gastric ulcer patients.The positive rate of Ure gene is 31.4% and the positive rate of CagA gene is 20.0% in the surface layer gastritis patients.The positive rate of Ure gene is 20.0% and the positive rate of CagA gene is 53.3 % in the atropic gastritis patients.Conclusion:Different Hp can cause different clinical manifestation of gastritis.