木素过氧化物酶降解偶氮染料卡布龙红的动力学及过程模型

KINETICS AND PROCESS MODELS FOR DEGRADATION OF AZO DYE CARPLON RED BY LIGNIN PEROXIDASES

研究了用含有木素过氧化物酶活力的酶液降解偶氮染料卡布龙红的动力学和过程 .在实验基础上 ,提出木素过氧化物酶降解卡布龙红的反应机理 ,并推导出了降解卡布龙红的酶反应速率方程 .应用该速率方程 ,考虑H2 O2 的自消失和H2 O2 对酶失活的影响以及产物卡布龙红正离子自由基的反应 ,建立了酶液分批降解卡布龙红的过程模型 。

Lignin peroxidase (Lip) was produced by phanerochaete chrysosporium fermentation and the fermentation broth was used to degrade azo dye, Carplon red. The initial reaction rates of dye degradation by Lip were experimentally measured by on-line analysis with HP8452A UV/Vis spectrometer at different concentrations of H2O2 and Carplon red. The experimental results showed that the initial reaction rates were inhibited by high concentration Carplon red. A mathematical model considering both two-substrate reaction and substrate (dye) inhibition was proposed and a reaction rate equation was derived. The model simulations were in good agreement with the experimental data. In batch degradation process, automatic disappearance of H2O2 and Lip deactivation by H2O2 were observed, and the cation free radical of dye was converted into degradation products. Based on the above experimental results, a model was established to describe the Lip-catalyzed batch dye-degrading process. The results indicated that the model was able to simulate the experimental data, and the average deviation is less than 10%.