食用油烟对大鼠肺Ⅱ型细胞氧化损伤作用的研究

Study on the Oxidative Damage Effects in Rat Lung Type Ⅱ Cells Exposed to Cooking Oil Fume

目的研究食用油烟颗粒提取物 (COF)对大鼠肺Ⅱ型细胞的氧化性损伤作用并探讨其作用机制。方法用食用油烟颗粒提取物对大鼠肺Ⅱ型细胞进行12h染毒并用N_乙酰半胱氨酸 (NAC)进行30min干预 ,采用硫代巴比妥酸比色法和二硫代二硝基苯甲酸比色法测定细胞中丙二醛 (MDA)与谷胱甘肽 (GSH)含量。结果随COF染毒剂量的增加和染毒时间的延长 ,大鼠肺Ⅱ型细胞MDA生成量明显增加 ,GSH含量明显降低。NAC预处理可降低大鼠肺Ⅱ型细胞MDA的产生及GSH的消耗。结论食用油烟颗粒提取物可通过脂质过氧化与干扰GSH抗氧化防御系统对大鼠肺Ⅱ型细胞产生氧化损伤作用。

Objective To study possible mechanisms of oxidative damages in rat lung type Ⅱ cells induced by cooking oil fume (COF). Methods The rat type Ⅱ lung cells were pretreated with anti_oxidant N_acetylcysteine (NAC) for 30 miuntes and were exposed to COF for 12 hours. The contents of MDA and GSH were detected with thiobarbituric acid colorimetric assay and 5,5_dithiobis 2_nitrobenzoic acid colorimetric assay. Results The significant increase of the contents of MDA and significant decrease of the contents of GSH were observed in the rat lung type Ⅱ cells with the increase of exposure doses of COF and the prolongation of exposure time of COF. The pretratment of NAC could reduce the production of MDA and increase the contents of GSH of the cells. Conclusion The possible mechanisms of oxidative damages in rat lung type Ⅱ cells induced by cooking oil rume might be formation of lipid peroxidation and interference of GSH anti_oxidative systems of the cells.