聚碳酸六亚甲基酯-共-癸二酸酐的合成及其体外生物降解性能

Synthesis and in vitro Biodegradation of Poly(hexamethylene carbonate-co-sebacic anhydride)

将齐聚碳酸六亚甲基酯二酸和癸二酸按照 2 5∶ 75、5 0∶ 5 0和 75∶ 2 5 (质量比 )的比例共聚 ,合成出含有脂肪聚碳酸酯片段和癸二酸酐 (SA)的聚碳酸六亚甲基酯 -共 -癸二酸酐 (PHCSA) ;PHCSA的结构经FTIR和 1 H NMR证实 .PHCSA在 0 .1 mol/L、p H7.4磷酸盐缓冲溶液中的降解速率随着 SA含量的增加而增大 :降解 1周后 ,SA摩尔分数分别为 80 %、5 8%和 3 2 %的 PHCSA,其失重率分别为 64.3 %、45 .2 %和2 1 .5 % ;PHCSA的降解为酶所催化 :SA摩尔分数分别为 5 8%和 3 2 %的 PHCSA在含有 1 %脂肪酶的缓冲溶液中降解 1周后 ,其失重率分别为 5 1 .2 %和 2 6.1 % ;PHCSA降解样内外层的分子量存在明显的差异 :降解2周后 ,SA摩尔分数分别为 80 %、5 8%和 3 2 %的 PHCSA,其内外层分子量与初始分子量之比 Mw/Mw0 分别为89.9%和 2 7.9%、98.1 %和 3 9.1 %以及 97.8%和 47.2 % ;这些结果说明

Poly(hexamethylene carbonate co sebacic anhydride)s(PHCSA) containing aliphatic polycarbonate and labile anhydride groups was synthesized by copolymerization of diacid derived from oligo(hexamethylene carbonate)diol(OHCMD) with sebacic acid(SA) in mass ratios of 25∶75, 50∶50 and 75∶25. The structure of PHCSA was confirmed by FTIR and 1H NMR. The in vitro degradation of PHCSA was performed in 0 1 mol/L phosphate buffer saline(PBS, pH 7 4) at 37 ℃. It was found that the degradation rate of PHCSA increased with the content of SA. After one week degradation, the mass loss of PHCSA contained 80%, 58% and 32%(mole fraction) SA were 64 3%, 45 2% and 21 5% respectively. It was also found that the degradation of PHCSA could be catalyzed by lipase. After one week degradation in 1% lipase/PBS(0 1 mol/L, pH 7 4) at 37 ℃, the mass loss of PHCSA contained 58% and 32%(mole fraction) SA were 51 2% and 26 1% respectively. There was an obvious difference between the inner and the outer layer of samples in degradation. After two week degradation, the M w/ M w0 (ratio of final molecular weight to the initial molecular weight) of the inner layer and the outer layer of PHCSA contained 80%, 58% and 32%(mole fraction) SA were 89 9% and 27 9%, 98 1% and 39 1%, 97 8% and 47 2% respectively.