水稻细菌性条斑病菌分离菌及其近缘种类血清学关系的研究

SEROLOGICAL RELATION DETECTION OFXANTHOMONAS CAMPESTRIS PV. ORYZICOLAAND THEIR NEAR RELATION

为了解四川省水稻细菌性条斑病菌(Xanthomonas campestris pv.oryzicola)及其近缘种菌株间的血清学关系,用浓度为10^(10)个细菌/ml的X.c.pv.cryzicola悬浮液免疫家兔,得效价为1/2560的抗血清,在琼脂平板上以孔径Amm、孔间距7mm对释释8倍的抗血清进行琼脂双向扩散试验,获自四川省的68个X.c.pv、cryzicola分离菌均产生完全吻合的沉淀线,证明属同一血清型;X.c.pv.oryae、与X·leersiae呈分技沉淀线,证明它们有血清关系但不是同一血型,与X.c.pv.o-ryzaeX.c.pv.citri以及X.c.pv.campestris不发生沉淀反应,它们无血清关系.

ODD were used between X.c.pv.oryzicola and X.c.pv.oryzae, X.c.pc.citri, X.c.pv.camp-estris, X.leersiae. The antigen and antiserue diffused in agearmedia in A appropriate concentration. The diameters of the wells were 4mm and the distance between two wells was 7mm. The results showed 68 X.c.pv.oryzicola strains in Sichuan got accordant precipitate lines and were a same serological type. X.c.pv.oryzae, X.c.pv.citri, X.c.pv.campestris got no precipitate lines and were different type. But between X.c.pv.oryzicola and X.leersiae got cross-reaction and have serological relation partly