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载反义MCP-1纳米粒子的制备及应用 被引量：4

Preparation and using of anti - MCP - 1 - loaded nanoparticles
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摘要 目的研究纳米粒子作为基因载体的可能性。方法采用高速搅拌溶剂挥发法制备载反义MCP-1的纳米粒子,观察其粒径,表面形态,含药量,包埋效率,体外释放,应用于血管再狭窄动物模型的效果。结果载反义MCP-1纳米粒子可有效抑制动物模型血管内膜增生程度。结论 PLGA纳米粒子可作为基因载体。 Objective To Study the feasibilty of gene - loaded nanoparticles. Methods Polylactic acid copolymer ( PLGA) nanoparticles containing anti - MCP - 1 were formulated with oil - in water high speed beat emulsion/solvent evaporation technique. Experiments were conducted to characterize the properties of the nanoparticles, the in vitro release of gene - nanoparticles, and the effection of anti - MCP - 1 loaded nanoparticles.Results Anti - MCP - 1 gene nanoparticles were proved to be efficient in restraining the hyperplasia of intima of blood vessel when infused direcdy to the bollon injured vein.Conclusion PLGA nanoparticles can be use as gene delivery.
作者 杨菁 宋存先 王彭延 李拥军 管珩
机构地区 中国医学科学院/中国协和医科大学生物医学工程研究所 北京协和医院
出处 《医学研究通讯》 2002年第4期11-14,共4页 Bulletin of Medical Research
基金 国家自然科学基金
关键词 纳米粒子 生物降解 基因载体 高速搅拌溶剂挥发法 术后血管再狭窄 基因治疗 动脉粥样硬化 Nanopaiticles Bicxlegradable gene delivery
分类号 R654.206 [医药卫生—外科学]
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  • 1平其能.现代药剂学[M].北京:中国医药科技出版社,1999..
  • 2Lebedeva I,Benimetskaya L,Stein CA, et al. Cellular delivery of antisense oligonucletides[J]. Eur J Pharm Biopharm, 2000,50(1):101-119.
  • 3Griffith TS, Chin WA, Jackson GC, et al. Intracellular regulation of TRAIL-induced apoptosis in human melanoma cells[J]. J Immunol,1998,161:2833-2840.
  • 4Petak I, Douglas L, Tillman DM, et al. Pediatric rhabdomyosarcoma cell lines are resistant to Fas-induced apoptosis and highly sensitive to TRAIL-induced apoptosis[J]. Clin Cancer Res, 2000, 6: 4119-4127.
  • 5Soppimath KS, Aminabhavi TM, Kullkarni AR, et al. Biodegradable polymeric nanoparticlees as drug delivery devices [J]. J Contr Rel, 2001,70(1-2):1-20.
  • 6Crommelin DJA, Storm G, Jiskoot W, et al. Nanotechnological approaches for the delivery of macromolecules[J]. J Contr Rel,2003,87(1): 81-88.
  • 7Liu WG, Yao K D. Chitosan and its derivatives--a promising non-viral vector for gene transfection[J]. J Contr Rel,2002, 83(1): 1-11.
  • 8Bannerman DD, Tupper JC, Ricketts WA, et al. A constitutive cytoprotective pathway protects endothelial cells from lipopolysaccharide-induced apoptosis[J]. J Biol Chem, 2001,276(18):14924-14932.
  • 9Vila A, Snchez M,Tobío M, et al. Design of biodegradable particles for protein delivery [J].J Contr Rel,2002,78(1-3):15-24.
  • 10Perez C, Sanchez A, Putnam D, et al. Poly(lactic acid)-poly(ethylene glycol) nanoparticles as new carriers for the delivery of plasmid DNA[J]. J Contr Rel,2001,75(1-2):211-224.

引证文献4

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医学研究通讯
2002年 第4期

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