PCR法检测慢性乙肝患者外周血单个核细胞中HBV-DNA

DETECTTON OF HVB DNA IN MONONUCLEAR CELLS IN PERIPHERAL BLOOD OF HEPATITIS B PATIENTS BY PCR ASSAY

目的 :了解慢性乙型肝炎患者外周血单个核细胞 (PBMCs)HBV的感染情况及其与血清HBV的关系。方法 :采用聚合酶链反应 (PCR)检测 5 5例慢性乙型肝炎患者PBMCs和血清中HBV。结果 :PBMCsHBV -DNA检出率为 69% (3 8例 /5 5例 )。血清HBV -DNA检出率为 3 6% (2 0例 / 5 5例 ) ,PBMCsHBV -DNA与血清HBV -DNA无一致性 ,P <0 0 5 ,PCR法检测慢性乙型肝炎患者HBV -DNA、PBMCs检出率优于血清检出率 ,P <0 0 5。结论 :在慢性乙型肝炎患者中 ,PBMCs存在HBV -DNA。

Objective: To investigate the hepatitis B virus (HBV) infection in peripheral blood mononuclear cells (PBMCs) and sera of hepatitis B patients. Method:Polymerase chain reaction (PCR) assay was used to detect HBV DNA in PBMCs and sera from 55 patients with chronic hepatitis B.Result: The detected positive rate of HBV DNA was 69% in PBMCs and 36% in sera. There not was correlation between HBV DNA in PBMCs and in sera. The detected rate of HBV DNA in PBMCs was superior to that in sera by PCR assay. Conclusion: There is HBV DNA in PBMCs and its detected positive rate is higher than that in serum by PCR assay.