急性胰腺炎大鼠肝脏核因子κB活化与肝损伤的关系

Relationship between hepatic NF-kB activation and liver injury in rats with acute pancreatitis

目的:观察急性胰腺炎大鼠肝脏中核因子κB(nuclear factorkappa B,NF-κB)的活化及其与肝损伤之间的关系。 方法:Wistar大鼠72只,随机分为3组:急性胰腺炎组(AP),急性胰腺炎PDTC处理组(APP),假手术组(SO)。分别于术后3,6,12,24h检测肝组织中NF-κB活性、血浆丙氨酸氨基转移酶(ALT)、淀粉酶(AMS)水平,并观察肝脏及胰腺病理改变。 结果:S0组术后未见NF-κB活化,血浆ALT及肝脏病理无显著变化。与SO组相比,AP组术后3～6hNF-κB活性显著增加(3.13±0.57 vs 0.71±0.10,1.92±0.26 vs 0.67±0.11,P<0.01),血浆ALT在3～24h也显著高于SO组(1.1±0.2 vs 0.7±0.09,1.6±0.2 vs 0.7±0.1,2.4±0.4 vs 0.7±0.09,3.5±0.7sv 0.8±0.1,P<0.01),肝组织病理改变明显重于SO组。在运用了NF-κB抑制剂PDTC的APP组,NF-κB活性显著低于AP组(1.85±0.38 vs 3.13±0.57,1.36±0.22 vs 1.92±0.26,P<0.01),血浆ALT较AP组也有了显著下降(1.2±0.2 vs 1.6±02,1.7±0.2 vs 2.4±0.4,2.1±0.4 vs 3.5±0.7,P<0.01或P<0.05)肝脏病理改变较AP组也有明显减轻。 结论:急性胰腺炎大鼠肝组织中存在着NF-κB的显著活化,活化的NF-κB参与了肝损伤的发生。

CONCLUSION: Hepatic NF-icB is significantly activated during acute pancreatitis. The activation of NF-kB involves in the liver injury.AIM: To learn the relationship between hepatic NF-kB activation and liver injury in rats with acute pancreatitis.METHODS: Seventy-two wistar rats were randomly divided into three groups: acutepancreatitis group (AP), acute pancreatitis treated with pyrrolidine dithiocarbamate (PDTC) group (APP) and sham operation group (SO). The hepatic NF-kB activity and the level of ALT and AMS in serum were detected at 3 h, 6 h 12 h and 24 h. Meanwhile, the pathological changes of liver and pancreas were observed with light microscope.RESULTS: The activity of NF-KB were significantly higher in AP group than that in SO group from 3 to 6 hours (3.13 ± 0. 57 vs0.71±0.10, 1.92±0.26 vs0.67±0.11, P<0.01),and the level of serum alanine aminotransferase (ALT) were significantly higher in AP group than that in SO group from 3 to 24 hours (1.1 ±0.2 vs 0.7±0.09, 1.6±0.2 vs 0.7±0.1, 2.4±0.4 vs 0.7±0.09, 3.5±0.7 vs 0.8±0.1, P<0.01). Meanwhile, the pathological changes were more severe in AP group than that in SO group. However, the activity of NF-KB were significantly lower in APP group than that in AP group( 1.85±0.38 vs 3.13±0.57,1.36±0.22 vs1.92±0.26, P< 0.01). ALT were also significantly lower in APP group than that in AP group( 1.2±0.2, vs 1.6±0.2, 1.7±0.2 vs 2.4 ±0.4, 2.1 ±0.4 vs 3. 5 ±0.7, P < 0. 01 or P < 0. 05). Compared with AP group, the hepatic pathological changes were significantly alleviated in APP group.