高效液相色谱法测定异烟肼的血药浓度

Assay of Concentration of Isoniazid in Plasma With HPLC

目的 :建立测定血浆中异烟肼浓度的高效液相色谱法。方法 :色谱柱 :EclipseXDBC18(4 6mm×150mm ,5μm ) ;流动相 :0 05mol/L磷酸二氢铵 -乙腈 (65∶35)。0 5ml血浆用10 %三氯醋酸沉淀蛋白后 ,加桂皮醛衍生化 ,再用乙醚提取分离后进样。结果 :在0 10～12 0μg/ml范围内线性关系良好 ;日内误差小于7 % ,日间误差小于14 % ;方法平均回收率95 %～105 %。结论 :本方法具有快捷、灵敏度较高、干扰少等特点 。

OBJECTIVE:A HPLC method has been developed to determine the concentration of isoniazid in plasma.METH_ODS:The Eclipse XDB-C18 column was used as fix phase and acetonitrile-0.05mol/L ammonium dihydrogen phosphate as mo_bile phase,detection wavelength was 280nm.The plasma sample was injected directly for determination after being deproteinized with 10% trichloroacetic acid and reacted with cinnamaldehyde and abstracted with ether.RESULTS:Good linear relationship was shown from 0.10 to 12.0μg/ml and the averge recovery of isoniazid was 95%～105%.CONCLUSION:The method is rapid,sensitive and is rarely interfered so it can be used in study of pharmacokinetics of isoniazid.