子宫内膜异位症与人类白细胞抗原DRB1等位基因多态性的关系

Study on polymorphism of human leukocyte antigen-DRB1 allele in patients with endometriosis

目的 探讨子宫内膜异位症 (内异症 )与人类白细胞抗原 (HLA) DRB1等位基因之间的易感相关性。方法 采用聚合酶链反应 序列特异性引物法 ,对 4 0例内异症患者 (内异症组 )及 5 0例正常女性 (对照组 )的HLA DRB1等位基因位点进行分类。结果 内异症组和对照组共检出 13种DRB1等位基因。内异症组等位基因检出率最高者是DRB1 15 ,检出率为 4 5 0 % (18/40 ) ,等位基因频率为 0 2 5 84 ;对照组等位基因DRB1 15的检出率为 2 6 0 % (13/5 0 ) ,等位基因频率为 0 1398,两组比较 ,差异有显著性 (P <0 0 5 )。在DRB1 15等位基因中共检出 15 0 1和 15 0 2两个多态性位点。内异症组中DRB1 15 0 1阳性率为 35 0 % (14 /40 ) ,等位基因频率为 0 1938,DRB1 15 0 2阳性率为10 0 % (4/40 ) ,等位基因频率为 0 0 5 13;对照组中DRB1 15 0 1阳性率为 2 0 0 % (10 /5 0 ) ,等位基因频率为 0 10 5 6 ,DRB1 15 0 2阳性率为 6 0 % (3/5 0 ) ,等位基因频率为 0 0 30 5。内异症组与对照组DRB1 15 0 1和DRB1 15 0 2两个多态性位点比较 ,差异无显著性 (P >0 0 5 )。结论 内异症的发生与HLA DRB1 15有关 。

Objective To study the possibility of a correlation between occurrence of endometriosis (EM) and polymorphism of human leukocyte antigen (HLA) DRB1 allele Methods Polymorphism of HLA DRB1 allele were detected by polymerase chain reaction瞫equence specific primers (PCR SSP) testing 40 surgically proven EM patients and 50 normal control Results At HLA DRB1*15 allele, positive frequency of EM group was significant higher as compared with those of the normal control group [45 0% vs 26 0%, P <0 05, relative risk ( RR )=2 1] Other HLA DRB1 allele positive frequency of EM group and control group had no significant difference But for Polymorphism of DRB1*15 allele, positive frequency of EM group and control group had no significant difference [1501∶35 0% vs 20 0%; 1502∶10 0% vs 6 0%; P >0 05] Conclusions The occurrence of EM may be associated with the presence of HLA DRB1 allele, but hasn′t associated about polymorphism of DRB1*15 allele HLA DRB1*15 maybe one of some factors for the pathogenesis of endometriosis