反相高效液相色谱法测定血浆中文拉法辛浓度

Determination of venlafaxine in plasma by RP-HPLC

目的 :建立高效液相色谱法检测人血浆中文拉法辛浓度。方法 :血浆样品经石油醚 乙醚 (2∶1 )提取后 ,有机相再用50mmol·L- 1盐酸反萃取并浓缩进样。色谱柱为氰基柱 (2 50× 4.6mm ,5μm) ,乙腈 -0 .1mol·L- 1NH4 H2 PO4 (2 5∶75)为流动相 ,UV检测波长 2 2 9nm。结果 :文拉法辛血浆最低检测浓度 1 0 μg·L- 1,线性范围 2 5～ 80 0 μg·L- 1,萃取回收率 75.5%～79.3%,加样回收率 97.4%～ 1 0 1 .2 %,日内RSD 4.87%～ 6 .39%,日间RSD 7.55%～ 1 0 .80 %。结论 :该法准确可靠 ,已用于临床患者文拉法辛血药浓度测定。

OBJECTIVE To establish an RP-HPLC method for the determination of venlafaxine in human pl asma.METHODS The plasma samples were extracted by the mixture of petroleum ether and ether(2 ∶1).The organic layer seperated was back-extracted by 50 mmol·L -1 HCl. T he chromatographic separation was carried out on CYANO column by using acetonitr ile- 0.1 mol·L -1 NH 4H 2PO 4 (25∶75) as the mobile phase. The dete ction wavelength was 229 nm.RESULTS The detection limit was 10 μg·L -1 in plasma.The standard curve was linea r within the range of 25～800 μg·L -1 .The recovery of extraction was 7 5.5% ～ 79.3% .The recovery of methodology was 97.4% ～ 101.2% . The RSD for within-day and between-day were 4.87% ～ 6.39% and 7.55% ～ 10. 80% ,respectively.CONCLUSIONS This method is accurate and reliable,it has been used for the determination of venlafaxine in the clinical trial.