一组高效稳定纤维素分解菌复合系MC1的筛选及功能

Selection of A Composite Microbial System MC1 with Efficient and Stability Cellulose Degradation Bacteria and Its Function

从 4种堆肥样品中分别筛选出纤维素分解能力较强的 4组混合菌 ,再以酸碱反应互补的原则重新优化组合并驯化成 1组纤维素分解能力非常强而稳定的纤维素分解菌复合系MC1 .1 0 0mL培养物在 5 0℃静止培养 72h ,分别可分解 0 48g滤纸、0 49g脱脂棉、0 1 9g麦秆和 0 0 8g锯末 .分解滤纸时 ,第 2 4hCMC糖化力最高 ,为1 2 2 3U·mL- 1.在初始pH4～ 1 0的不同培养基上接种 ,均能把 pH调到中性 ,最后稳定在 8 0～ 8 5之间 .在连续投放滤纸情况下纤维素分解能力稳定保持 2 0d以上 ,其发酵液的 pH在有滤纸时稳定在 6 0～ 6 5之间 ,没有滤纸时稳定在 8 0～ 8

Four groups of microbial mix culture with considerable capability of cellulose degradation were selected from four compost heaps,by combining the four groups,a composite microbial system MC1 with highly efficient cellulose degradation was obtained.It was found that 0 48g of filter paper,0 49g of absorbent cotton,0 19g of wheat straw,or 0 08g of wood residue can be degraded by 100mL of MC1 at 50℃ with in 72 hours under static culture.The CM1 saccharification activity was much higher at 24th hours when degrading filter paper that was 122 3U·mL -1 .This capability of degradation maintained more than 20days when the substrates were continually added.MC1 could be inoculated in a wide pH rang,from 4 to 10;however,the final pH would be changed to neutrality after incubation.The pH would be stability with filter paper between 6 0 and 6 5,and between 8 0 and 8 5 without the filter paper.