硒与铅的致突变性及硒的拮抗效应

Mutagenicity of Selenium and Lead and Antagonistic Effects of Selenium on Lead

目的 探讨硒与铅的致突变性及硒的拮抗效应。方法 给予ICR小鼠经口灌胃不同浓度的亚硒酸钠（Na2SeO32.5、5.0和10mg/kg）和醋酸铅[Ph（Ac）2 5、10和25mg/kg],连续3d,进行骨髓嗜多染红细胞微核计数。结果 单纯10mg/kg Na2SeO3、10、25mg/kg Pb（Ac）2组微核率均高于对照（P<0.01）;硒（2.5、5.0和10mg/kg）-铅（10 mg/kg）联合染毒Ⅰ组显示5.0mg/kgNa2SeO3组微核率（2.7‰）低于2.5mg/kgNa2SeO3组（5.0‰）和10mg/kgNa2SeO3组（3.2‰）及单纯10mg/kg Pb（Ac）2组（6.6‰）,P<0.01,但仍高于阴性对照（2.3‰）,P<0.01;硒（2.5、5.0和10mg/kg）-铅（25mg/kg）联合染毒Ⅱ组显示5.0mg/kg Na2SeO3组微核率（2.9‰）低于2.5mg/kgNa2SeO3组（6.4‰）和10mg/kg Na2SeO3组（3.8‰）及单纯25mg/kg Pb（Ac）2组（7.9‰）,P<0.01,但仍高于阴性对照组。结论 硒和铅均具有一定的致突变性,一定量的硒对铅的遗传毒性有拮抗效应。

ve To explore the mutagenicity of selenium and lead, the antagonistic effects of selenium on lead. Methods The ICR mice were orally perfused with sodium selenite (Na2SeO3) at different concentrations of 2.5,5.0 and 10 mg/kg and lead acetate [Pb(Ac)2] at different concentrations of 5,10 and 25 mg/kg jointly and con-tinuously for 3 days, and then the numbers of bone marrow polychromative erythrocytes were counted. Results The frequencies of micronucleus of mice only exposed to 10 mg/kg Na2SeO3, 10, 25 mg/kg Pb(Ac)2 respectively showed higher levels compared with those of control (P<0.01). Selenium (2.5,5.0 and 10 mg/kg)-lead (10 mg/kg) combi-nation exposure group Ⅱ showed: the frequency of micronucleus of 5.0 mg/kg Na2SeO3 group (2.7‰) was lower than that of 2.5 mg/kg Na2SeO3 group (5.0‰), 10 mg/kg Na2SeO3 group (3.2‰) and single 10 mg/kg Pb(Ac)2 group (6.6‰), P<0.01, and was higher than that of negative control group (2.3‰) . Selenium (2.5,5.0 and 10 mg/kg) -lead (25 mg/kg) combination exposure group Ⅱ showed: the frequency of micronucleus of 5.0 mg/kg Na2SeO3 group (2. 9‰) was lower than that of 2. 5 mg/kg Na2SeO3 group (6. 4‰), 10 mg/kg Na2SeO3 group (3. 8‰) and single 25 mg/ kg Pb(Ac)2 group (7.9‰), and was also higher than that of negative control. Conclusion Selenium and lead both presented mutagenecity in a certain degree. Selenium at some dose revealed antagonestic effects on lead-induced mutagenicity.