锰对人外周血淋巴细胞微核率和姊妹染色单体交换频率的影响

Effects of Manganese on Frequencies of Micronucleus and Sister Chromatid Exchange in Human Peripheral Blood Lymphocytes in Vitro

目的 探讨锰对正常人外周血淋巴细胞微核率和姊妹染色单体交换（SCE）频率的影响。方法 用不同浓度的硫酸锰溶液（培养液中锰浓度:0.05、0.15、0.25、0.75、1.50μg/ml）处理体外培养的人外周血淋巴细胞,观察其微核率和SCE频率的变化,同时以生理盐水为对照。结果 锰剂量≤0.25μg/ml时,实验组与对照组相比,其微核率和SCE频率变化均不显著（P>0.05）;锰剂量≥0.75μg/ml时,微核率和SCE频率均显著高于对照组（P<0.01）,且呈剂量-反应关系。结论0.25μg/ml以下低剂量锰对细胞遗传物质是比较安全的,而0.75μg/ml以上高剂量锰可造成不同类型DNA损伤。

ve To study the effects of manganese on frequencies of micronucleus and sister chromatid enchage(SCE) in peripheral blood lymphocytes of healthy human in vitro. Methods The frequencies of micronucleus and SCE were detected in human peripheral blood lymphocytes cultured in vitro. The lymphocytes were incubated with manganese sulfate solution with various concentrations of manganese in incubation solution of 0.05, 0.15, 0.25, 0.75 and 1.50μg/ ml in experimental group, and normal saline in control group. Results No significant differences were observed in frequencies of micronucleus and SCE between experimental group with manganese doses of ≤0.25 μg/ ml, and control group (P>0.05) . Significantly higher frequencies of micronucleus and SCE were observed in experimental group with manganese doses of ≥0.75 μg/ml compared with that of control group (P> 0.01). A sig-nificant dose-reponse relationship was also found between the manganese exposure doses and the frequencies of mi-cronucleus and SCE in experimental group. Conclusion Lower exposure levels of manganese of ≤0.25μg/ ml were safe to genetic materials, but higher manganese exposure levels of ≥0.75μg/ml might cause different kinds of DNA damages.