25例急性髓性白血病初治患者白血病细胞端粒长度分析

The analysis of telomere length in acute myelogenous leukaemia

目的 探讨急性髓性白血病患者端粒长度的变化及意义。方法 采用Southern杂交方法测定端粒长度。结果 AML患者平均TRF长度 (7.6± 2 .1kb)较正常对照 (9.3± 1.9kb)明显缩短。结论 端粒的丢失是急性髓性白血病恶性转化中的一个重要的遗传学改变 ,端粒的丢失可能导致染色体的失稳 ,在白血病的发生中起着重要的作用。

Objective To study the changes and significance of telomere lenghth in acute myelogenous leukaemia. Methods The method of Southern hybridization was used to estimate the length of telomere. Results Mean terminal restriction fragments (TRF) length of AML (7.6±2.1 kb) was significantly shorter than that of normal control(9.3±1.9 kb). Conclusion It suggests that the lose of telomere is a important change of genetics of AML in the process of malignant transformation .It may lead to chromosomal instability and play an important role in the occurrence and development of AML.