长毛对虾卵母细胞环形片层的研究

Studies on Annulate Lamellae in Oocytes of Prawn Penaeus penicillatus

应用电镜技术研究长毛对虾(Penaeus penicillatus)卵母细胞中的环形片层(AL)。AL在卵黄发生前的卵母细胞卵质中出现,在卵黄发生期间消失。AL由8条平行排列,具有50～70 nm 环孔的片层组成。相邻片层之间的距离约为70 nm。AL 通常没有核糖体附在膜片层的外表面。AL末端膨大,与高尔基体毗邻。从形态学上看,AL可能来源于高尔基体;高尔基膜囊伸直、拉平,并局部融合形成环孔,最后演变成AL。卵黄发生期间,AL分化形成为许多囊泡,为卵黄粒的形成提供沉积的场所。本文还讨论高尔基体、AL 及卵黄粒形成之间的关系。

Using electron microscopy, annulate lamellae in oocytes of P. penicillatus have been investigated. The annulate lamellae usually appear to be emerging in ooplasm at provitellogenic stage and disappearing at vitellogenic stage. The annulate lamellae are consisted of a stack of about 8 lamellae parallelly arranged and interrupted by the presence of many pores about 50-70 nm in diameter. The distance between adjacent lamellae is quite uniform that is about 70nm. The annulate lamellae usually do not have ribosomes attached directly to the outer surface of the membranes. The ends of annulate lamellae are dilated and next to Golgi bodies. Morphologically, it is possible that annulate lamellae are derived from Golgi bodies. The cisternae of Golgi bodies appear to stretch, bring to same level, fuse and form pores in some areas, finally change into annulate lamellae. During vitellogenesis, annulate lamellae appear to differentiate and form into many vesicles that may provide places for the formation of yolk granules.The ralationship between Golgf bodies, annulate lamellae and the formation of yofk granules has been discussed in this paper.