西洛他唑对糖尿病大鼠黏附分子的影响

Effect of cilostazol on adhesion molecules of STZ-induced diabetic rats

目的 探讨西洛他唑对糖尿病 (DM)大鼠黏附分子CD54、CD10 6、CD62p的表达和坐骨神经传导速度及对肾脏以及主动脉病变的影响。方法 实验设正常组 8只大鼠 ,糖尿病组 8只大鼠 ,胰岛素组 7只大鼠和西洛他唑组 7只大鼠。测定各组坐骨神经传导速度 ,单个核细胞表面CD54、血小板表面CD62p以及肾脏、主动脉CD54或CD10 6的表达水平。结果 西洛他唑明显加快坐骨神经传导速度 (DM组 2 0 3m·s-1± 2 2m·s-1,西洛他唑组 2 8 9m·s-1± 7 9m·s-1,P <0 0 5 )。降低单个核细胞表面CD54的表达 (DM =6 5 1%± 14 9% ,西洛他唑组 2 5 4 %± 8 6 % ,P <0 0 5 ) ,减少肾脏和主动脉CD54和CD10 6表达 ,改善肾脏和主动脉病理变化。而降低血小板表面CD62p的作用不明显 (DM组 4 0 4 %±8 7% ,西洛他唑组 2 8 5 %± 3 7% ,P >0 0 5 )。结论 西洛他唑能降低CD54、CD10 6的表达 。

Objective To investigate the effect of cilostazol on the expression of CD 54 , CD 106 , CD 62P in kidney, aorta, surface of platelet, and mononuclear cell of blood in STZ induced diabetic rats Methods Rats were randomly divided into four groups: normal control group( n =8), diabetes control group( n =8), insulin group( n =7, subcutaneous injection of insulin, 1～4 U·d -1 ), and cilostazol group( n =7, gastric infusion of cilostazol, 18 mg·kg -1 ·d -1 ) Sciatic nerve conductive velocity and level of CD 54 /CD 62p on the surface of mononuclear cell/platelet were examined by flow cytometry Immunohistochemistry was used to examine the expression of CD 54 and/or CD 106 in kidney and aorta Results The sciatic nerve conductive velocity was 20 3±2 2 m·s -1 and 28 9±7 9 m·s -1 in diabetic rats and cilostazol group respectirely ( P <0 05) The mononuclear cell surface expression of CD 54 was 69 1±14 9% and 25 9±8 6% in diabetic group and cilostazol group respectively ( P <0 05) The expression of CD 54 /CD 106 in kidney and aorta was decreased The expression of CD 62p on the surface of platelets was 40 4±8 7% in diabetic group and 28 5±3 7% in cilostazol group ( P >0 05) Conclusion Cilostazol treatment delays the development of diabetic pathological change in kidney, aorta and sciatic nerve and decreases the expression of CD 54 and CD 106