Fas及Fas-L在宫颈腺癌组织中的表达

Fas and Fas-L expression in human adenocarcinoma of uterine cervix

目的 探讨Fas及Fas L在宫颈腺癌中表达的意义。方法 36例宫颈腺癌组织及其癌旁正常宫颈腺上皮用免疫组织化学方法进行Fas及Fas L检测 ,DNA原位末端标记 (TUNEL)检测凋亡细胞。结果 TUNEL标记指数与肿瘤分化程度呈正相关趋势 ,低分化腺癌明显低于高、中分化腺癌(P <0 .0 5 ) ,高、中分化腺癌间差异无显著性 (P >0 .0 5 )。Fas在宫颈腺癌组织中的表达阳性率为86 .1% ,在癌旁正常宫颈腺上皮仅 3例有低表达 ,其余均为阴性 ;宫颈腺癌组织Fas表达强度与肿瘤分化程度呈负相关 (P <0 .0 5 )。Fas L在宫颈腺癌组织中的表达阳性率为 10 0 % ,在癌旁正常宫颈腺上皮仅 1例有低表达 ,其余均为阴性 ;Fas L的表达与肿瘤分化程度无显著相关性 (P >0 .0 5 )。结论宫颈腺癌Fas的表达与肿瘤的恶性程度呈正相关 ,Fas L的表达可能与肿瘤逃避免疫监视有关。

Objective To study the significance of Fas and Fas-L expression in adenocarcinoma of uterine cervix. Methods Both carcinoma tissue and their surrounding tissues from 36 patients with adenocarcinoma of uterine cervix, previously untreated either by radiation or chemotherapy , were studied for the expression of Fas and Fas-L by immunohistochemical stain with DNA apoptosis fragment detected by TUNEL. Results The TUNEL labeling index was negtively correlated with differentiation of adenocarcinoma of cervix . Compared to highly differentiated and moderately differentiated tumor,the TUNEL labeling index was reduced obviously in poorly differentiated adenocarcinoma ( P <0.01). Fas expression was detected in 31 cases(86%)while there were only 3 weakly stained in the normal endocervical glands around the carcinoma. The 5 unstained carcinomas were 3 highly differentiated and 2 moderately differentiated .The positively stained Fas was associated with differentiation; the stronger the stain, the less differentiation there was. The Fas-L expression was detected in all adenocarcinomas while there was only 1 weakly stained in the normal ones. No significant difference was found in the expression of Fas-L in carcinomas with different degrees of differentiation. No correlation was observed between Fas and Fas-L expression. Conclusions The Fas expression is positively correlated with the different degrees of differentiation and Fas-L expression may be associated with the escape from of immunal surveillance.