摘要
目的从乌头属植物中提取分离剧毒生物碱乌头碱、中乌头碱、次乌头碱;方法酸性水溶液提取,碱化后中性氧化铝柱层析,IR、EI-MS确认结构;结果利用该方法提取以上3种生物碱得到的回收率与碱性苯溶液法相似。结论该方法所用试剂成本低、毒性小,适用于制备乌头碱、中乌头碱、次乌头碱对照品。
Objective To establish a method for extracting and separating mesaconitine,aconitine and hypaconitine.Methods Aconitum kusnezoffii reichb was extracted with0.2mol/L HCl and adjusted to pH10.The precipitate was chromatographed over neutral alumina.Each alkaloid's structure was identified with IR and EI-MS.Results The recovery received from this method was similar with that of ammoniacal benzene method.Conclusion This method was at low cost,harmless and suitable for preparing mesaconitine,aconitine and hypaconitine.
出处
《刑事技术》
2002年第3期16-17,共2页
Forensic Science and Technology
关键词
乌头
生物碱
提取
分离
中毒检验
aconitum
aconitine
mesaconitine
hypaconitine
