少根根霉γ-亚麻酸固态发酵条件的研究

Studies on Solid State Fermentation Conditions of GLALipid fromRhizopus Arrhizus

通过He-Ne激光、紫外线和化学诱变剂LiCl复合诱变,获得高产GLA菌株少根根霉RC378,摇瓶培养含油脂量47.8%,GLA含量12.6%。经发酵条件优化得到菌株RC378固态发酵条件,培养基基本配比为∶麸皮∶玉米粉∶菜籽粕∶大米粉=6∶4∶2∶1,培养基料与水分比例为1∶1.5,在添加2%蔗糖,0.2%NH4NO3,0.4%柠檬酸钠,0.3%ZnSO4的情况下,采用孢子悬液接种,发酵前期(72h)30℃培养,后期(60h)24℃培养其固态发酵干基油脂含量可达9.4%～12.9%,GLA含量可达9.8%～12.6%。

AmutantstrainRC378accumulating a large amountof lipidwas successful ly obtainedas treatedby U.V.,He -Ne laser and LiCl to induce compou nd mutagen.The content of lipid in RC378cultivated in shaking flask was47.8%.The content ofGLAinLipidwas12.6%.After studying onlipidprodu cing conditions ofRC378,the optimu m fermentation conditions for lipid p roduction were:Wheat bran∶Corn pow der∶Repeseed residue∶Rice flour =6∶4∶2∶1.Withtheaddingof2%sucrose,0.2%NH 4 Cl,0.4%sodiumcitricacid,and 0.3%ZnSO 4 ,theratioofsubstrateand water was 1∶1.5,inoculatedwithsuspensionspore.Inthefirst72h,itwa s cultivatedat30℃,andintheother60hitwascultivated at24℃.The content of lip idwas kept at9.4%～12.9%,and GLAkep t at9.8%～12.6%.