固定化米曲霉菌体填充床拆分DL-丙氨酸的动力学行为

Kinetics of Mycelium-immobilized Packed Bed Continuous Resolution of DL-alanine

分析了固定化米曲霉拆分DL-丙氨酸的动力学特性,通过间歇反应器的实验数据处理,获得了酶促反应的动力学参数. 应用填充床的一维稳态轴向扩散模型进行数值求解,得到了模型计算值,与实验结果比较表明,提出的模型能较好地描述固定化菌体连续拆分DL-丙氨酸过程.

Based on the systematical study of continuous resolution of DL-alanine employing the immobilized Aspergillus oryzae cell, the kinetics of the enzymic reaction was determined. Furthermore, the method of Newton-Raphson was applied to the solution of one-dimension-steady-diffusion model of packed bed for continuous resolution of DL-alanine. The calculated results of the model, with the error less than 10%, were in good agreement with those of experiments. It was demonstrated that the model was reasonable and accurate.