胚芽中雌激素成分的GC/MS测定

Determination of Entrin Plumule by GC/MS

胚芽是妊娠三个月以内流产的子宫内容物 ,制成干粉装入胶囊后用于不孕症。经KCL液浸泡、乙醚提取 ,提取物经乙酰化衍生后在GC -MS上进行雌激素成份鉴定 ,实验结果表明胚芽每克干粉中含雌激素 0 .5 94mg ,其中雌酮、雌二醇、雌三醇的百分率 (% )分别是 2 7.6 ,6 8.1,4 .3。

Plumule is the content of uterus after abortion during pregnancy three months.IT was made into dry powder to treat infertility.The dry powder was soaked in KCL solution,extracted with ether and then was acetylated.Finally Estrin in Extraction was determinated by GC/MS.The results show that each gram of dry powder contains estrin 0.594 mg which includs Estrone(E 1),Estradiol(E 2) and Estriol(E 3):27.6,68.1,4.3(%)separately.