间苯二酚分光光度法测定芥菜多糖中的果糖含量

Resorcinol-spectrophotometric Method for the Determination of Fructose in Mustard Leaf′s Amylose

本论文利用Seliwanoff反应 ,在乙醇介质内测定了芥菜多糖中果糖的含量。果糖与间苯二酚之间生成的红色络合物在 4 73nm处吸收峰最高 ,在 0～ 83μg·mL- 1 范围内符合比耳定律 ,A =0 0 2 8+0 0 2 0 2 2c ,r =0 9998。测得芥菜多糖中含有 11 4 1%的果糖。

A method using seliwanoff reaction is proposed for the determination of fructose in the amylose of mustard leaves. Fructose reacts with resorcinol forming a red compound with a maximum absorption at 473 nm. The calibration curve is linear over the range of 0-83 mug . mL(-1) with a correlation coefficient of 0.999 8. The content of fructose in the amylose of mustard leaves is found to be 11.41%. Quntitative analysis used for the fructose of syrupus include such as volumetric analysis, then layer chromatography, polarimetry, high pressure liquid chromatography and spectrophotometric analysis. According to different color-developing agents, spectrophotometric analysis may be classified as carbazole method, ammonium molybdate way and resorcinol way etc. reported by foreign papers is hard to operate and colored complex is unstable.