摘要
综述了低温X射线晶体衍射法在生物大分子结构测定中的发展与应用，介绍了在数据收集中的急速降温，冷冻防护剂的使用和载晶技术, 以及低温X射线晶体衍射法应用于测定生物反应中间体的最新进展。
Cryocrystallography is an important technique in the area of biological structural determination. The development of cryocrystallography is reviewed, and a new application of cryocrystallography in the studies of intermediate is introduced.
