流动注射化学发光法测定DL-酪氨酸

Determination of DL-tyrosine by flow injection analysis with chemiluminescence method

在甲醛存在下 ,高锰酸钾与DL 酪氨酸能够发生化学发光反应 ,产生很强的化学发光。据此采用流动注射技术 ,建立了一种测定DL 酪氨酸的化学发光分析法。方法的检出限为 2 .9× 1 0 - 8g/mL ,相对标准偏差为 1 .5 % ( 1 .0× 1 0 - 6g/mLDL 酪氨酸 ,n =1 1 ) ,线性范围为 1 .0× 1 0 - 7g/mL～ 5 .0× 1 0 - 6g/mL。

Chemiluminescence reaction of DL tyrosine with potassium permanganate in the prescence of formaldehyde as an enhancer was investigated.A new chemiluminesence method for the determination of DL tyrosine with flow injection technique was developed.The detection limit was 2.9×10 -8 g/mL with relative standard deviation of 1.5%(1.0×10 -6 g/mL DL tyrosine, n =11),and the liner range of 1.0×10 -7 g/mL～5.0×10 -6 g/mL.