人源化甲状腺素脱碘酶单链抗体的结构模拟

Computer Simulation of Human Iodothyronine Deiodinase Single-chain Antibody

以四碘甲状腺素原氨酸为半抗原 ,从人噬菌体展示半合成单链抗体库中筛选与半抗原有特异结合性的单链抗体 E3 ,并测定了 E3的一级结构 。

Iodothyronine plays a major role in growth, basic metabolism and organ formation. It has an extremely limited source in the body. In this thesis, we designed iodothyronine(T4) as hapten. Then a single chain antibody displayed on phange was obtained from a human phage displaying a single chain antibody library. The specific genes of E3 was subcloned in P 5E vector. According to its amino acid sequences, we simulate its three dimention structure by computer. It has never been reported in PDB.