中草药提取物体外抑制HBV的筛选实验

Inhibiting Effects of The Extacts From Herbs on Human Hepatitis B Virus in vitro

目的 :以乙型肝炎病毒 (HBV)DNA转染的细胞株 2 2 15细胞为靶细胞 ,给药后通过检测细胞培养液中HBsAg和HBeAg滴度 ,对四种中药抗HBV效果进行评价。 方法 :在 2 2 15细胞培养基中分别加入不同浓度的药物 ,培养数天后检测上清液中HbsAg和HBeAg滴度 ,计算药物对DNA表达的抑制率 (ID50 ) ;用四甲基偶氮唑盐 (MTT)比色分析法检测药物对细胞的毒性 ,计算出半数中毒计量 (LD50 ) ,用治疗指数 (TI)判断药物的抗HBV病毒作用。结果 :槲寄生和甘草的水提取物有较好的抗HBV作用 ;芫花提取物则有较大的细胞毒性。白薇提取物的抗HBV病毒作用不显著。结论 :本实验方法作为快速体外筛选模型简便易行 。

Objective: The inhibiting effects of extracts from Radix et rhizoma nardostachyos, Viscum xorloratum Nakai, Flos genkwa, Radix cynanchi atrati on human HBV in vitro were evoluated by 2.2.15 cell line. Method: To establish the new assay method, the culture medium of 2.2.15 cell line was injected the drugs. Then the value of HbsAg or HbeAg was detected. The toxin to the cell can be tested by MTT test. The activity of the drugs was evoluated by TI. Result: The anti-HBV activity of Radix et rhizoma nardostachyos and Viscum xorloratum Nakai is showed obviously. Another drugs show the negative. Conclusion: The method is simple and availible. It is worthy of future investigation for studying about Radix et rhizoma nardostachyos and Viscum xorloratum Nakai.