消癌平对SGC-7901胃癌细胞的作用及机制的实验研究

The Mechanism of laboratory research for xiaoaiping treating SGC 7901 gastric carcinoma cellular strains

目的 探讨消癌平治疗胃癌的肿瘤细胞抑制作用的机制。方法 1.观察不同浓度消癌平对SGC 790 1胃癌细胞的抑制率并进行体外抑制试验 ;2 .取对数生长期的SGC 790 1细胞 ,浓缩体积到 1ml,先后加入纤维蛋白原和凝血酶工作液使之形成细胞凝块 ,并将其切成等体积小块 ,将瘤体置于昆明种水鼠肾囊下。移植后次日开始采用消癌平 0 .4,0 .6 ,0 .8ml/kg浓度腹腔内注射 ,连续 7d。注射后第 8天处死小鼠 ,于显微镜下测量肿瘤的各径 ,以计算肿瘤体积和肿瘤抑制率 ,探讨对人胃癌SGC 790 1细胞生长的抑制作用 ;3.采用流质细胞仪观察消癌平对人体胃癌SGC 790 1细胞株的抑制机制。结果 1.体外抑制试验 :消癌平 5 0 ,40 ,30 ,2 0 ,10mg/ml浓度时对SGC 790 1胃癌细胞的抑制率为 10 0 % ,5 9 % ,2 9% ,19%和 15 % ,与空白对照组差异明显。其高浓度的抑制率与HCPT(98% )相仿 ,药物作用 7d后的IC50 为 2 1mg/ml。 2 .昆明种水鼠胃癌细胞株移植后 ,采用的消癌平为 0 .4,0 .6,0 .8ml/kg ,腹腔内注射的肿瘤抑制率为 5 0 % ,5 3% ,71%。Logit法计算消癌平对荷瘤小鼠的ED50 为 0 .44 /kg ,t检验表明各用药剂量组的抑瘤率与空白对照组比较有显著差异 ,高剂量时消癌平的抑瘤率 (71% )与阳性对照组5 Fu的抑瘤率 (76% )相似。

Objective On the purpose of assessing inhibition efficacy and mechanism of Xiaoaiping (XAP, a kind of Chinese medicine) for SGC 7901 gastric carcinoma cellular strains.Methods 1. Extracorporal inhibition experiments were undertaken for SGC 7901 gastric carcinoma cellular strains using XAP 50,40,30,20,10mg/ml concentrations respectively. 2. SGC 7901 gastric carcinoma cellular strains on the logarithmic growth periods(4×10 7 cells) were concentrated to 1ml in volume. It was coagulated after mixing with thrombin and fibrin and then the coagulated clot was implanted in renal capsules of Kunming water mouse(certifications: No.005, CSAC) for intracorporal experiments. After continual injecting Xiaoaiping intra abdominal cavity was seven days, with 0.4,0.6,0.8ml/kg concentrations respectively. On the 8th day all the water mice were sacrificed using eyepicec ruler for calculating tumor size under microscope including inhibition hate. 3. In order to pursue mechanism of Xiaoaiping on gastric carcinoma, analyses were carried out on FACSTar with cellular analysis of cellfit DNA analysis software, after Xiaoaiping(30mg/ml) mixed with SGC 7901 cellular strains(1×10 6 cells) seven days. Simultaneously Giemsa decoration method as used for observing tumor cell morphologic changes. Results 1. Extracorporal inhibition rates were 100%,59%,29%,19% and 15% to SGC 7901 gastric carcinoma cells using XAP 50,40,30,20,10mg/ml concentrations respectively. Obvious differences were found in comparison with control groups, inhibition rate of XAP in high concentration is similar with HCPT(98%), IC 50 concentration was 21mg/ml after used XAP for seven days. 2. Tumor inhibition rates were 50%,53% and 71% respectively with continual injecting Xiaoaiping into abdominal cavity seven days by 0.4,0.6,0.8ml/kg concentrations. ED 50 was 0.44/kg for Kunming Water Mouse by logit method. Obvious differences were found in comparison with control groups. Inhibition rate(71%) of XAP in high concentration reached favorabler standard with 5 Fu (76%). 3. DNA analyses showed that cellular period were 65.70% in G 0 G 1 period, 16.22% in G 2 M period, 18.09% S period respectively. G 2/G 1 ratio was 1.86 after XAP(30mg/ml) mixed with SGC 7901 cellular strains seven days, the ratio of cellular nucleus to syrup was reduced as tumor cell morphology. Conclusion Xiaoaiping appears to have good inhibition effect on gastric carcinoma cellular strains and should be as an alternative choice for treatmenting gastric carcinoma and metastases.