TIPSS术中胆汁漏出对内皮细胞NOS活性影响的研究

Bile leakage in transjugular intrahepatic portosystemic shunt: effect on endothelial NOS activity

目的 探讨经颈静脉肝内门腔分流术 (TIPSS)中胆汁漏出对内皮细胞一氧化氮合酶(NOS)及一氧化氮 (NO)合成的影响 ,进一步了解胆汁对支架内皮化的影响。方法 取人脐静脉内皮细胞进行体外培养 ,加入不同浓度 (5 %、10 %、15 %、2 0 %、2 5 % )胆汁干预 ,观察内皮细胞生长状况 ,并测条件培养液中NO及细胞NOS活性。结果 含 5 %、10 %、15 %胆汁的细胞生长状况与不含胆汁者相似 ,含 2 0 %、2 5 %胆汁的细胞明显减少并显幼稚 ;各浓度胆汁的细胞NOS活性较不含胆汁者明显降低 ;条件培养液中NO含量均无明显差异。结论 胆汁抑制内皮细胞的生长 。

Objective To evaluate the effect of bile on endothelial nitric oxide synthase(NOS) activity. Methods Endothelial cells explanted from human umbilical vein were cultured in vitro, then bile of different concentrations 5%,10%,15%,20%,25% were mixed with the standard culture medium. Viability and morphology of the endothelial cells were studied, and NO(nitric oxide) content in conditioned medium and NOS activity were assessed. Results Viability and morphology of endothelial cells in the culture mediums presented as 5%,10%,15% bile were similar to the cells without bile medium while endothelial cells in mediums presented with 20% and 25% bile were markedly reduced in number and immature. Endothelial cell NOS activities in mediums presented with various concentration of bile were statistically decreased comparing with the cells in medium without bile. The quantity of NO in conditioned culture mediums with or without bile did not reach a statistical significance. Conclusion Bile appears to inhibit endotholial cells growth as well as endothelial NOS activity.