c-fos在电针调控大鼠胃运动中的表达及其意义

Expression of c-fos Gene and Its Significance in Effects of Electroacupuncture on Gastric Motor in Rats

目的 :探讨原癌基因c fos在穴位电针对胃运动影响中的表达及其意义。方法 :采用免疫组织化学方法及电生理的方法 ,观察电针刺激“足三里”等不同穴位 ,c fos在中枢延髓的孤束核(NTS)及迷走神经背运动核 (DMV)中的表达 ,同时采用浆膜法检测胃电变化情况。结果 :电针刺激“足三里”等不同穴位c fos在NTS及DMV中的表达情况不同 ,且胃电也发生较为明显的变化。结论 :穴位电针对胃运动具有调节作用。以c fos的表达作为激活标志 。

Objective:To observe c fos gene expression and analyze its significance in electroacupuncture (EA) regulation on gastric motor in rats. Methods:64 SD rats were randomly divided into 'Zusnali' (ST 36) group (n=8), 'Shangjuxu' (ST 37) group (n=8), non acupoint group (n=8) and control group (n=8). By using immunohistochemical technique (ABC method), c fos gene expression in the Dorsal motor nucleus of the vagus nerve (DMV) and nucleus tractus solitary(NTS) was displayed. After anesthesia (846 mixture, 0.1 mL/100 g body weight), the rat's abdominal cavity was opened and an induction electrode was implanted at the serous coat of the anterior wall of the stomach (about 0.3 ～0.5 cm to the pylorus) for recording EGG(electrogastrography). Left 'Zusanli'(ST 36) and 'Shangjuxu'(ST 37) were stimulated with EA by setting the stimulating parameters being frequency of 50 Hz, voltage of 20 V and duration of 30 min. Results:In control group, c fos positive neurons in DMV and NTS were few. After EA of 'Zusanli'(ST 36) and 'Shangjuxu'(ST 37), c fos positive neurons increased considerably in number in comparison with those of control group (P<0.01). While those of 'Zusanli'group were obviously more than those of 'Shangjuxu' group (P<0.01) but no significant difference was found between non acupoint group and control group (P>0.05). Simultaneously, changes (increase of both frequency and amplitude after EA) of EGG of the four groups presented the same tendency. Conclusion: EA can adjust gastrtic motor function, which may be closely associated with EA activating neurons of NTS and DMV.