“气至病所”对相关皮瓣浸泡液内5-羟色胺、肾上腺素、去甲肾上腺素含量的影响

The Effect of"Qi Zhi Bing Suo"on the Contents of 5-hydroxytryptamine, Norepinephrine and Epinephrine in the Skin-flap Soak Liquid

为研究“气至病所”的外周神经体液机制 ,本课题借用福尔马林试验 ,在大鼠一侧足底皮下注射 5%福尔马林 50 μL作为局部病灶 ,观察电针“阳陵泉”、“外丘”穴 (刺激参数为 1～ 3V ,4～1 6Hz,3 0min)对大鼠自发痛行为反应、相关皮瓣浸泡液内 5 羟色胺 ( 5 HT)、肾上腺素 (E)、去甲肾上腺素 (NE)含量的影响。结果表明 ,大鼠一侧足底注射福尔马林后 0～ 80min出现明显的自发痛行为反应 ,电针能显著减少大鼠福尔马林试验后的自发痛行为反应 ;大鼠一侧足底注射福尔马林后60min相关皮瓣浸泡液内 5 HT、NE、E含量显著增加 ,电针能拮抗大鼠福尔马林试验后相关皮瓣浸泡液内 5 HT、NE、E含量的上升。结果提示 ,循经电针可通过外周神经体液机制促使“气至病所” 。

Objective:To research the peripheral neurohumoral mechanism of 'Qi Zhi Bing Suo'In the rat. Methods: Thirty Wistar male rats were randomly divided into normal saline (NS) group (n=10), formalin (F) group (n=10) and electroacupuncture (EA)+ F group (n=10) for observing pain behavior changes ; other 40 Wistar male rats were divided into NS, EA+NS, F and EA+F groups with 10 rats in each group. In these rats of each group, subcutaneous injection of NS 50 μL or 5% formalin 50 μL was performed in rats' plantar center respectively for inducing local formalin focus and saline control. EA of'Yanglingquan'(GB 34) and 'Waiqiu'(GB 36) on the focus side was given to the rats of EA group with stimulation parameters of 1～3 V,4～16 Hz and 30 min for observing the analgesic effect. The contents of 5 hydroxytryptamine (5 HT),Norepinephrine (NE) and Epinephrine (E) in the skin flap soak liquid were detected using fluorospectrophotometry. Results: 0～ 80 min after subcutaneous injection of formalin, the evoked spontaneous pain behavior (shortening leg, licking or biting the red swelling focus) appeared, while after EA the number of leg shortening and biting duration decreased significantly (P<0.05). Sixty minutes after subcutaneous injection of formalin, the contents of 5 HT, NE and E in the skin flap soak liquid all increased significantly in comparison with those of NS and EA+NS groups (P<0.05～ 0.01), while those of F group were significantly higher than those of EA+F group, indicating an inhibitory effect of EA on release of 5 HT, NE and E from the skin flap. Conclusion: The results suggest that EA of acupoints could promote'Qi Zhi Bing Suo' (qi reaching the focus) along the focus related meridian via peripheral neurohumoral mechanism and reduce release of local inflammatory mediators and lighten hyperalgesia caused by sympathetic efferent.