不同剂量丹参注射液抗肝纤维化的实验研究

Experimental Research of the Various Doses Treatment Groups of salvia miltiorrhiza to Prevent Liver Fibrosis Induced with CCI_4

目的:探讨不同剂量丹参注射液对四氯化碳(CCl_1)所致中毒性肝纤维化的防治作用及各剂量间疗效的比较。方法:采用CCl_4制造大鼠肝纤维化模型,同时用高、中、低剂量丹参注射液治疗,测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(A1b)、透明质酸(HA)、重型前胶原(PCⅢ)、Ⅳ型胶原(ⅣC),并以光镜观察组织学改变,综合分析不同剂量丹参注射液对CCl_4中毒性肝纤维化的防治作用。结果:高、中、低剂量丹参注射液都有降低ALT、AST的作用,并有显著降低HA、PCⅢ、ⅣC的作用。经治疗后的肝细胞损伤、肝脏脂肪变性与纤维组织形成较少。其中高、中剂量组的疗效比低剂量组为好,而高、中剂量组两者之间的差异无显著意义。结论:本实验提示丹参高、中剂量组防治肝纤维化的作用优于低剂量组。

To investigate the effect of the various doses treatment groups of salvia miltiorrhiza on liver fibrosis(LF) induced with CCl4 Method:The treatment groups of salvia miltiorrhiza were divided into high doses group, medium doses group and low doses group. The different doses treatment groups were compared with model group and control group, and the three treatment groups compared the effect with each other. Serum level of ALT, AST, albumin, HA, ⅣC, PCⅢand pathological changes of hepatic tissues were analyzed. Results: Serum level of ALT, AST, HA, ⅣC,PC Ⅲ in the treatment groups were obviously lower than model group and the pathological results under microscope were much better than model group. The effect of high doses group was similar to the medium doses group and these two groups were much better than the low doses group of treatment. Conclusion: The results showed that the effect of high and medium doses groups were better than the low doses group.