用^(45)Ca研究中药的钙拮抗作用及机理

Study on Ca^(2+) Antagonistic Effect and Mechanism of Chinese Herbal Drugs Using ^(45)Ca

用^(45)Ca跨膜测量技术研究了中药及治疗冠心病中药复方对大鼠主动脉平滑肌细胞膜Ca^(2+)通道的影响。结果表明:西红花、川红花、地奥心血康等对平滑肌细胞膜电压依赖Ca^(2+)通道(PDC)和受体操纵Ca^(2+)通道(ROC)有阻滞作用,其中以西红花作用最强;中药复方中全方与各功能药均不影响静流(Leak),对ROC、PDC均有明显阻滞作用。藿香与佩兰均是以正己烷提取组分的钙拮抗作用最强;藿香的活性成分有5个,佩兰的活性成分有2个。

The Ca2+ antagonistic effect and mechanism of Chinese herbal drugs are studied by 45Ca. The results indicate that potential-dependent Ca2+ channel (PDC) and receptor-operated Ca2+ channel (ROC) in cell membranes of smooth muscle can be blocked by vseveral Chinese herbal drugs, including as Crocus sativus L. , Carthamus L. , Di-ao-xin-xue-kang(DAXXG) and Ginkgo biloba L. leaves. Among them Crocus sativus L. has the strongest antagonistic effect on Ca2+ channel, while Ginkgo biloba L. leaves has no obvious effect. The whole prescription and the other functional drugs have significant effect on ROC and PDC. The compositions extracted by hexane have the strongest antagonistic. The wrinkled giant hyssop have five active compositions and Pei-lan have two active compositions.