血管内皮生长因子对日本和牛胚胎卵裂率和4～8细胞期发育率的影响

Effect of Vascular Endothelial Growth Factor on the Rate of Cleavage and Development to 4- to 8-cell embryos in Vitro of Japanese Black Oocyte

为了探明血管内皮生长因子对IVM/IVF/IVC生产的日本和牛胚胎的卵裂率和到 4～ 8细胞期胚胎发育率的作用 ,在IVM/IVF/IVC的过程中 ,分别在SOF成熟液、BO液以及SOF培养液中添加 0、0 .1、1、10ng/ml的VEGF。通过对5 33个胚胎的检测得出 ,添加VEGF对胚胎卵裂率和 4～ 8细胞期发育率有促进作用 ,其卵裂率分别从对照的 4 7.3%提高到 5 1.4 % (0 .1ng/ml,P >0 .0 5 )、72 .6 0 % (1ng/ml,P <0 .0 5 )和 6 8.5 % (10ng/ml,P <0 .0 5 ) ;4～ 8细胞期发育率则分别从32 .0 %提高到 36 .4 %、5 9.7% (P <0 .0 5 )、5 2 .9%。结果证明 :VEGF对IVM/IVF/IVC的日本和牛胚胎发育有积极的促进作用。可以提高胚胎的卵裂率和 4～ 8细胞期胚胎发育率 。

To examine the effect of Vascular Endothelial Growth Factor (VEGF) on the cleavage rate and development rate to 4 to 8-cell stage of Japanese Black oocyte, human recombinant VEGF 165 was supplemented at the different concentration of 0, 0.1, 1 and 10ng/ml during in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) in the present experiement. Cumulus oocyte complexes (COCs) were matured for 22 h in modified Synthetic Oviduct Fluid (m-SOF) supplemented with 10% fetal bovine serum (FBS), inseminated for 6 h in BO medium and cultured in 1% FBS-m-SOF. The cleavage rate was improved from 47.3% of the control to 51.4%, 72.6%(P<0.05) and 68.5%(P<0.05) in 0.1, 1 and 10 ng/ml VEGF groups respectively. The development rate to 4 to 8-cell stage was increased to 36.4%, 59.7%(P<0.05) and 52.9% from 32.0%(control) in 0.1, 1, 10 and 0ng/ml(control) groups respectively. These results indicate that VEGF has a beneficial effect on the development of Japanese Black oocyte.