肉牛生长激素基因Hae Ⅲ座位多态性与主要生产性能的相关研究

Study on An HaeⅢ Polymorphism at the Bovine Growth Hormone Gene Associated with Main Performance

本研究利用PCR RFLPs标记技术 ,对 6 3头杂种肉牛的生长激素基因的第 5外显子和 3’端区域进行了分析研究 ,发现HaeⅢ酶切座位具有多态性 ,并分为三种基因型 (即AA、BB和AB型 ) ,通过构建最小二乘线性模型 ,分析了生长激素基因型与杂种肉牛 12月龄体重和 6 - 12月龄日增重的关系。结果表明 :生长激素AB基因型的效应稍高于AA、BB型 。

Using PCR-RFLPs to analyse polymorphism of bovine growth hormone(bGH) gene. We have discovered polymorphism of locus in the fifth exon and 3′flanking region of the bGH gene with the restriction enzyme HaeⅢ. Analysis the association between genotype of bGH and yearling weight, 6～12 month daily gain with Least Square analysis method. The results show effect of AB genotype is higher than that of AA and BB, but the effect different is not significantly between genotypes (P>0.05).