DNA免疫防制鸡马立克氏病的初步研究

Study on DNA Immunization and Control of Marek's Disease

将MDVgB基因插入真核表达质粒pcDNA3 CMV启动子下游多克隆位点,构建成重组真核表达质粒pcDNA3-gB。将重组真核表达质粒分2组进行雏鸡腿部肌肉注射。雏鸡进行一免,2周后一组用重组真核表达质粒再加强免疫一次,另一组用构建的重组鸡痘病毒（rFPV）加强免疫。2周之后攻毒,观察免疫保护效果。2次用重组真核表达质粒免疫组的免疫保护率为75％;而先免疫重组表达质粒,后免疫rFPV组的免疫保护率仅为50％。结果表明,MDVgB基因DNA免疫可以诱导鸡体产生免疫保护,但rFPV不能对其加强免疫。

gB gene of Marek' s disease virus was inserted into pcDNA3 multiple clone site at downstream of CMV promoter, forming eukaryotic expression recombinant piasmid pcDNA3 - gB. Immune test groups were inoculated with pcDNA3 - gB intramuscularly, two weeks later, one group were boosted with the same pcDNA3 - gB, the other group with recombinant fowlpox virus expressing gB(rFPV). At 4 weeks after priming, both group chickens were challenged with vMDV. Immune effects showed that the immune protection rate of two groups reached 75%, 50% .The results proved that pcDNA3-gB DNA immunization elicited immune protection, but rFPV can't boost immunization.