反相离子对高效液相色谱法分离不同种类的磷脂酰胆碱

Separation of Different Phosphatidyl Cholines by Reversed-Phase Ion-Pair High Performance Liquid Chromatography

采用反相离子对高效液相色谱 (RP IP HPLC)法分离分析了不同种类的磷脂酰胆碱 (PC) ,色谱柱为PERKIN ELMER/HS 5C18柱 ,流动相为甲醇 乙腈 水 (70∶2 2∶8,体积比 ) (内含 15mmol/L四甲基磷酸铵离子对试剂 ,pH 7) ,流速 2mL/min ,在 2 0 8nm波长处检测 ,该法成功地分离了

A reversed phase ion pair high performance liquid chromatographic (RP IP HPLC) method for the separation of different phosphatidyl cholines (PC) was established. The optimized conditions were a PERKIN ELMER/HS 5 C 2?8 column (150 mm×4 6 mm i d , 5 μm), isocratic elution with mobile phase of methanol acetonitrile water (70∶22∶8, volume ratio) containing 15 mmol/L tetramethyl ammonium phosphate (TMAP) at pH 7 0, flow rate of 2 0 mL/min and UV detection at 208 nm. Seven kinds of PC could be separated with this method.