高效液相色谱法测定注射用赖氨匹林中的阿司匹林及游离水杨酸

Determination of Aspirin and Free Salicylic Acid in Lysinipirine Injection by High Performance Liquid Chromatography

建立了高效液相色谱法 (HPLC)同时测定注射用赖氨匹林中阿司匹林和游离水杨酸含量的方法。采用的色谱柱为HypersilBDSC18柱 ,流动相为甲醇 水 冰醋酸 (体积比为 35∶6 5∶3) ,检测波长为 2 80nm。阿司匹林和水杨酸的质量浓度分别为 0 0 2 8g/L～ 0 14 1g/L和 0 77mg/L～ 3 85mg/L时线性关系良好 ,其线性相关系数分别为0 9999和 0 9998;加样回收率分别为 99 2 7% (RSD =0 8% )及 99 6 1% (RSD =1 3% )。

The contents of aspirin and free salicylic acid in lysinipirine injection were determined by high performance liquid chromatography (HPLC). A Hypersil BDS C 18 column was used with the mobile phase of methanol water acetic acid (35∶65∶3, volume ratio) and the detection wavelength of 280 nm The average recoveries of aspirin and salicylic acid added were 99 27%(RSD=0 8%) and 99 61%(RSD=1 3%), respectively The calibration curves had good linearity in the range of 0 028 g/L-0 141 mg/L and 0 77 mg/L-3 85 mg/L, and the correlation coefficients were 0 999?9 and 0 999?8 for aspirin and salicylic acid respectively.