非淋菌性泌尿生殖道感染支原体检测及药敏试验

Detection of Mycoplasma in 296 Patients with Non-gonococcal Urogenital Tract in Fection and Test of Susceptibility of Ureaplasma Urealytica to Antimicrobial Agents

目的 :了解解脲脲原体 (Uu)和人型支原体 (Mh)在非淋菌性泌尿生殖道感染中的致病作用及其体外药物敏感状况。方法 :2 96例疑为非淋菌性泌尿生殖道感染的患者 ,运用微量稀释法进行支原体培养 ,测定解脲脲原体对 10种抗菌药物的敏感性。结果 :10 1例 (3 4.1% )支原体培养阳性 ,其中Uu阳性 75例 (2 5 .3 % ) ,Mh阳性 6例 (2 .0 % ) ,Mh +Uu阳性 2 0例 (6.8% ) ,女性 (4 2 .9% )支原体阳性率显著高于男性 (2 8.8% )。Uu对螺旋霉素均耐药 ,对氧氟沙星、四环素的耐药率均为 3 7.3 % ,对交沙霉素的耐药率最低 (8.0 % ) ,对其他抗菌药物的耐药率依次为美满霉素 (3 3 .3 % )、强力霉素(3 2 .0 % )、红霉素 (3 0 .7% )、左氧氟沙星 (18.7% )、阿奇霉素 (13 .3 % )、罗红霉素 (10 .7% )。Uu对四环素类、大环内酯类和喹诺酮类有交叉耐药 ,以对四环素类和喹诺酮类的交叉耐药率最高 (12 .0 % )。结论

Objective:To explore the role of Ureaplasma urealytica (Uu) and Mycoplasma hominis (Mh) in non gonococcal urogenital tract infection and examine their in vitro drug susceptibility. Methods:Detection of mycoplasma and susceptibility test of ureaplasma urealytica to 10 antimicrobial agents were performed by using microdilution method. Results:Among the 296 suspected cases, 101(34.1%) were positive with mycoplasma , 75 (28.8%) with Uu, 6(2.0%) with Mh, and 20 (6.8%) with Mh plus Uu. The positive rate with mycoplasma infection was significantly higher among the female subjects (42.9%) than that among the male (28.8%). The drug susceptibility test showed that, among the 10 antimicrobial agents tested, resistance of Uu was most commonly found to spiramycin (100.0%), and least found to josamycine (7.9%). Frequency of resistance of Uu, among the tested cases, to the other antimicrobial agents, was as followes: to ofloxacin (37.3%), to tetracycline (37.3%), minocycline (33.3%), doxycycline (32.0%), erythromycine (30.7%), levofloxacin (18.7%), azithromycin (13.3%), and to roxithromycin (10.7%). The cross resistance to fluoro quinolones , tetracycline and macrolides was found. The highest rate of cross resistance was between fluoro quinolores and tetracycline (12.0%). Conclusion:Monitoring the variance of resistance of mycoplasma is of important significance for guiding the use of drugs.