结核患者各种体液中利福平浓度及其相关性研究

Determination of the Concentration of Rifampicin in Body Fluid of Patients with Tuberculosis by HPLC

目的 :建立高效液相色谱法测定结核病患者血浆、胸水、腹水利福平浓度的方法 ,进行药物浓度相关性研究。方法 :对 3 4例住院患者血浆、胸水、腹水中利福平浓度测定结果进行分析 ;高效液相色谱法测定采用C1 8色谱柱分离 ,波长在 3 3 3nm处检测 ,柱温 2 0℃。结果 :线性关系良好 (r =0 .9993 ) ,日内、日间RSD均 <10 % ,能满足测定要求。服药 2h后利福平浓度 ,血浆中最高 ,胸水、腹水中较低 ,而 12h后血浆中药物浓度在最低检测浓度以下 ,胸水、腹水中浓度仍远大于最低抑菌浓度 (0 .0 2 μg·mL 1 )。结论 :服药后 2h ,血浆与胸水及腹水浓度呈一定正相关 ;每日空腹使用利福平 1次 。

Objective:To establish a method for the determination of rifampicin concentration in the body fluid of patients with tuberculosis (TB) by HPLC, and study correlation of the concentrations in different body fluids. Methods:The rifampicin concentrations in plasma and the pleural fluid and ascites were determined by HPLC method in 34 patients with TB. Results:The rifampicin concentrations in plasma and the pleural fluid as well as ascites were linearly correlated ( r =0.999 3). RSD of intra and inter day were less than 10%. The rifampicin concentration in plasma peaked in the 34 patients in 2 hours after administration of rifampicin. At 12 hours after administration of rifampicin, the concentrations in plasma, pleural fluid as well as ascites were still higher than the MIC (0.02 μg·mL 1 ). Conclusion:The rifampicin concentrations in the pleural fluid and ascites were significantly correlated with that in the plasma. Administration of Rifampicin once daily could maintain its concentration at effective level at the target sites in the body.