维生素C对肺泡巨噬细胞脂质过氧化及抗氧化酶的影响

The effect of Vitamin C on lipid peroxidase and antioxidase enzyme of pulmonary alveolar macrophage

目的 探讨维生素C拮抗镍所致细胞毒作用及作用机理。方法 采用体外细胞培养方法,观察维生素C对染镍肺泡巨噬细胞包膜脂质过氧化产物丙二醛(MDA)及超氧化物歧化酶(SOD)活性的影响。结果 在体外染镍肺泡巨噬细胞培养过程中加入不同浓度维生素C(25,50和100μmol/L)可提高肺泡巨噬细胞内SOD活性,具剂量反应关系。维生素C能显著抑制MDA生成量,其中以50和100μmol/L浓度组作用最为明显。结论 镍可致细胞脂质过氧化,维生素C具有拮抗镍所致细胞毒性的作用,可能与其抗氧化功能有关。

[Objective] To study the effects of vitamin C on alveolar macrophage injury exposed to Ni2O3. [Methods] The alveolar macrophage was cultured in vitro with exposure to Ni2O3. Meanwhile, vitamin C(25,50 and 100 μoml/L) were added in the media respectively. The detection of malondialedlyde and activity of antioxidant enzyme in alveolar macrophage were carried out. [Results] Vitamin C could increase the activity of superoxide dismutase in alveolar macrophage with a dose - dependent manner. Both the vitamin E 50 μoml/L group and 199 μoml/L group had dramatic inhibitory effect. Vitamin E could inhibit the production of malondialdegyde by alveolar macrophage. [Conclusion] Vitamin C could inhibit the cytotocity of Ni2O3 which probably due to its antioxidant property.