排毒养颜胶囊中大黄素的含量测定

Determination of Emodin in Paidu Yangyan Capusle by TLC-Scanning

目的 :测定排毒养颜胶囊中大黄素的含量。方法 :用石油醚 (30～ 6 0℃ ) -醋酸乙酯 -甲酸 (10∶ 2∶0 .2 )为展开剂 ,在硅胶 G薄层板上分离大黄素 ,采用双波长直线式扫描测定其含量 ,λS=4 5 0 nm,λR=6 5 0 nm。结果 :大黄素在 0 .1～ 0 .7μg范围内呈良好的线性关系 ,r =0 .9993,加样回收率为 10 0 .38% ,RSD =0 .87%。结论 :该法简便 ,准确 ,专属性强 ,重现性好 。

Objective:To determine the quantity of emodin in Paidu Yangyan Capsule.Methods:TLC-Scanning.Results:There was a good linear relationship in the range of 0.1～0.7 μg for Emodin, r = 0.999 3 . The average recovery was 100.38%, RSD was 0.87%. Conclusion:The method is simple, accurate, specific, reproducible, and can be usded for the quality control of Paidu Yangyan Capsule.