牙龈卟啉单胞菌对脐静脉血管内皮细胞产生sICAM-1的影响

Effect of porphyromonas gingivalis on soluble intercellular adhesion molecule-1 production by human umbilical vein endothelial cells

目的 :观察牙龈卟啉单胞菌 (Porphyromonasgingivalis,P .gingivalis)对人类脐静脉血管内皮细胞 (humanumbilicalveinendothelialcells ,HUVECs)产生可溶性细胞间粘附分子 - 1(solubleintercellularadhesionmolecule - 1,sICAM - 1)的影响。方法 :应用厌氧袋法培养P .gingivalis,并用其感染HUVECs,采用酶联免疫吸附实验 (enzyme -linkedimmunosorbentassay ,ELISA)测定培养上清中sICAM - 1的含量。结果 :HUVECs基础表达少量的sICAM - 1;P .gingivalis剂量依赖性增强HUVECs产生sICAM - 1蛋白的水平 ;紫外线、超声波和 6 5℃的热处理都不能抑制P .gingivalis的作用 ;sICAM - 1蛋白水平在P .gingivalis刺激后的 4h未见改变 ,增高的作用从刺激后的 8h开始 ,在 12h、16h和 2 0h继续增加。结论 :活的和灭活的P .gingivalis都剂量依赖性和时间依赖性地增强HUVECs产生sICAM - 1,P .gingivalis可能同样诱导牙龈血管内皮细胞表达sICAM - 1,升高的sICAM- 1可能参与调节牙周病炎症反应和免疫反应过程。

AIM :To observe the effect of Porphyromonas gingivalis ( P. gingivalis ) on soluble intercellular adhesion molecule-1 (sICAM-1) production by human umbilical vein endothelial cells (HUVECs). METHODS: Anaeropack method was employed to culture P. gingivalis and then HUVECs were co-cultured with P. gingivalis following by assaying the amount of sICAM-1 with enzyme-linked immunosorbent assay(ELISA)kit. RESULTS: P. gingivalis dose-dependently increased the production of sICAM-1 in HUVECs. The treatment of UV, sonication and heating did not inhibit the effects of P. gingivalis . The amount of sICAM-1 did not increase at 4h after P. gingivalis infection; elevated sICAM-1 was found at 8h, and then continued to increase at 12h, 16h and 20h after co-cultivation. CONCLUSION: P. gingivalis increased the production of sICAM-1 in HUVECs with a dose and time dependent manner. P. gingivalis might induce the gingival endothelial cells to express sICAM-1 and elevated sICAM-1 might be involved in the regulation of inflammatory and immunological reaction in periodontal disease.